Published Animal Studies
Leigh E. Colby, DDS, MBA
Purpose: The purpose of this study was to selectively ablate the entire volume of targeted third molar (3M) tooth buds in a pig model. This study demonstrates the potential for a less-invasive alternative to contemporary surgical techniques for removal of 3Ms.
Methods: The investigator developed a mandibular split-mouth animal model study design. The model used pigs because the animals’ 3M tooth buds are dimensionally similar to those of humans. The study sample consisted of 5 female Yorkshire-cross pigs at 20 weeks of age. The investigator delivered microwave energy thermal doses to thermocoagulate tooth bud tissues inside the bony crypts of targeted 3M toothbuds. Based on the bony crypt dimensions obtained from computed tomography scans of each subject, the microwave thermal dose was predetermined. The mandibles were dissected to visually compare thermocoagulated right-side 3M tooth bud tissues to left-side untreated controls.
Results: All 5 study animals were successfully treated. All 5 fully guided third molar tooth bud ablation (3TBA) procedures resulted in thermocoagulation of the entire volume of targeted 3M tooth bud tissues, with no visual evidence of damage to structures beyond the bony crypt.
Conclusions: The animal model developed for this study enabled the demonstration of a fully guided 3TBA protocol. The animal model and 3TBA procedure employed in this study appear to be appropriate for use in future long-term animal studies designed to demonstrate the efficacy of 3TBA for inducing molar agenesis.
Ⓒ 2022 American Association of Oral and Maxillofacial Surgeons
J Oral Maxillofac Surg 80:1522-1533, 2022
Leigh E. Colby, DDS, MBA
Purpose: Fully guided microwave tooth bud ablation has the potential to become a minimally invasive means for managing third molars in adolescent patients. If developed, this new modality could provide improved outcomes and reduced complications compared to traditional third molar management strategies. The purpose of this 28-day longitudinal characterization study was to determine if the healing response following fully guided microwave ablation of second molar tooth buds in juvenile pigs would result in the complete removal of targeted tooth bud tissues, molar agenesis, and no significant collateral tissue damage.
Methods: Investigators performed fully guided microwave ablation on 24 mandibular second molar tooth buds (#18 and #31) in seven-week-old pigs. Postablation healing assessment consisted of radiographic and histological evaluation of 3 subcohorts (consisting of 4 animals each) at 7-, 14- and 28-days post ablation. Controls were untreated, opposing maxillary second molar tooth buds. Neurological assessment was performed to determine if there was any detectible loss of inferior alveolar nerve function.
Results: Healing processes were nearly complete at 28 days post ablation. While one tooth bud was identified as partially ablated at 14 days post treatment, all treated tooth bud tissues were replaced with trabecular new bone formation by the end of this study. There was no detectible loss of inferior alveolar nerve function. The thermal dosing strategy used in this study appears to deliver prescribed ablation volumes and–within the context of this animal model–there was no detected collateral tissue damage.
Conclusions: The results of this study confirm the hypothesis that healing processes following fully guided tooth bud ablation resulted in removal of targeted tooth bud tissues, complete molar agenesis, and trabecular new bone growth at 28-days post treatment.
Ⓒ 2022 American Association of Oral and Maxillofacial Surgeons 2022 Published by Elsevier Inc. on behalf of the American Association of Oral and Maxillofacial Surgeons
J Oral Maxillofac Surg -:1-11, 2023